A comparative study on oncostatin M secretion by transplantable melanoma cells with regard to their biological properties and progression.

The authors have studied oncostatin M secretory activity of cells of two lines of transplantable melanoma with different biological properties. The level of OSM in melanoma cells supernatant was determined by ELISA test and OSM bioactivity was measured by using biotest line sensitive to OSM cytostatic activity--A 375 melanoma cells. OSM presence was confirmed in cellular lysates by western blot. It has been demonstrated that a spontaneous alteration of the transplantable melanotic melanoma line into an amelanotic line connected with tumour progression was accompanied by an increase of OSM release.